43. Ghosts 'N Goblins
Fun Fact: Arthur appears in Marvel vs. Capcom 3
Ghosts 'N Goblins for NES is a faithful arcade port of the action platformer developed by Capcom, and is considered one of the most difficult games published for the system. The player controls the heroic knight Arthur, who is trying to fight through a horde of various fantasy monsters in order to rescue the imaginatively-named Princess Prin Prin, who has been captured by Satan.
If Arthur gets hit by an enemy, his armor flies off and he is reduced to his boxer shorts. The second hit kills him. Not only are there plentiful opportunities for Arthur to be forcefully disrobed, but the player is also on a timer. If they take any longer than three minutes on a level, they die and have to start over again. This is what people mean when they invoke the phrase “That's game's Nintendo hard.”