44. Harley Quinn

GAME: Batman: Arkham Asylum (PS3/360, 2009)
Don’t get us wrong—Harley Quinn is one clown we’d let tickle our balloon any day of the week. But when we’re in the shoes of the world’s greatest detective, she’s nothing more than a nuisance with way too much makeup on. Hearing her voice over the PA system was more punishing then being locked in Arkham in the first place. We now fully understand why Joker treats her like a jumpoff...too much mouth and too little action. Hopefully Arkham City will let Catwoman take her out the old-fashioned way. And by "old-fashioned," we mean "with barely contained lesbian overtones."